Multifrequency Phase and Modulation Fluorometry
نویسندگان
چکیده
منابع مشابه
Multifrequency and Modulation Fluorometry 1 Phase
General Aspects Determination of characteristic fluorescence parameters i.e. spectral properties, quantum yields, polarizations, and lifetimes, is important in the study of excited states of atoms, molecules, and crystals and also for diagnostic purposes in the physical, chemical, biological, and medical sciences. The appeal of fluorescence methodologies lies in their intrinsic sensitivity and ...
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Calcium interaction with phospholipid membranes containing phosphatidic acid is studied by multifrequency phase fluorometry, using DPH as fluorescent molecule. DPH decay is analysed by a continuous distribution of lifetimes. The results suggest an increase of membrane heterogeneity at low calcium concentrations, without changes in the polarity of the environment surrounding the probe.
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Time-resolved decays of fluorescence anisotropy were obtained from frequency-domain measurements of the phase angle difference between the parallel and perpendicular components of the polarized emission and the ratio of the modulated amplitudes. These data were measured at modulation frequencies ranging from 1 to 200 MHz. To demonstrate the general applicability of this method, we describe the ...
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Time-resolved decays of fluorescence anisotropy were obtained from frequency-domain measurements of the phase angle difference between the parallel and perpendicular components of the polarized emission and the ratio of the modulated amplitudes. These data were measured at modulation frequencies ranging from 1 to 200 MHz. To demonstrate the general applicability of this method, we describe the ...
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We present here a comparative study on the decay of chlorophyll (Chl) a fluorescence yield in thylakoid membranes and photosystem II (PSII)-enriched samples, measured with multifrequency cross-correlation phase fluorometry. These measurements confirm the general conclusions of Van Mieghem et al. (Biochim. Biophys. Acta 1100, 198-206, 1992), obtained with a flash method, on the effects of reduct...
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ژورنال
عنوان ژورنال: Annual Review of Biophysics and Bioengineering
سال: 1984
ISSN: 0084-6589,0084-6589
DOI: 10.1146/annurev.bb.13.060184.000541